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Artificial Insemination Procedure

by Dr. Ray Favero
Date Posted: 01-01-2010

A method of insemination called cervical insemination is commonly used in whitetail deer.
This method of insemination is very similar to the method used to inseminate goats. Similarities exist between the goat and whitetail deer. Mainly both are seasonal breeders and have fairly high fertility.

It varies from operation to operation, but some producers keep the. does in stalls or smaller pens from the time of CIDR removal until artificial insemination. The most important thing is to keep stress to a minimum from the time of CIDR removal until the time of artifiical insemination.

Procedures like worming, vaccinating or weaning should be done before or at CIDR
insertion. If the does will be tranquilized for insemination, then feed and water should be removed for at least 12 hours prior to insemination.
The equipment required for insemination are:

-Semen and well filled nitrogen tank
-Thawing thermos with 95 degree water
-Insemination gun for one quarter or one half cc straws
-Scissorsor straw cutting devise
-Speculum with light source, smaller or larger is helpful depending on doe age
-Non-spermicidal lubricant
-Microscope and supplies (optional)
-Slide warmer (optional)
-Paper towels

Myself and many insemination technicians often preform a pre-insemination evaluation of
the thawed semen with the microscope prior to insemination. Whitetail semen can be quickly evaluated for gross motility, but differs from other species in that the semen must be warmed to near body temperature before any movement is seen. .This isin contrast to most other species where if the semen is a little cool, the movement is present but just a little slow. Every year several thousand dollars worth of whitetail semen is thrown away because an inexperienced and untrained technician looks at a cold microscope slide and says that the semen is dead. If the slide is warmed the semen will begin moving and is fine. But often this semen is discarded and causes quite a lot of hardship and financial loss.

After the straw is thawed in the 95 degree water, the straw is dried and the end of the
straw (non-cotton plug end) is cut. The straw is inserted into a warmed insemination gun and kept warm. The lubricated speculum is gently inserted into the vagina of the doe. A slight upward angle is used as the speculum is started into the females reproductive tract. If difficulty in introduction is encountered, then a smaller speculum should be used. The speculum is advanced up to the cervix. Using the light, the opening of the cervix is visualized. While looking down the speculum, the insemination gun is inserted through the speculum and into the opening of the cervix. Gentle forward pressure along with slight rotation and side to side movement aid in advancing the insemination gun further into the cervix. Sometimes the entire cervix is penetrated, but often the gun is moved through one to two inches of the cervix. Semen is deposited in this area, by depressing the plunger of the insemination gun.

After insemination the tranquilization is reversed or the does is released from the chute.
Experienced technicians can inseminate 12 to 15 per hour on tranquilized does or 20 to 25 does in a good drop floor chute.